Immunoprophylaxis of anthrax. Anthrax vaccine combined fluid (Vaccine anthracicum combined fluid) Instructions for use of anthrax vaccine

Cutaneously (scarification) and subcutaneously.
It is advisable to carry out unscheduled vaccination subcutaneously.
Primary immunization is carried out twice with an interval of 20-30 days, revaccination is carried out once annually. A cutaneous dose of the vaccine is 0.05 ml (contains 500 million spores), one subcutaneous dose is 0.5 ml (50 million spores).
Cutaneously (scarification): the contents of the ampoule immediately before use are resuspended in sterile 30% aqueous solution glycerol, which is added to the ampoule using a syringe with a needle for intramuscular administration. The volume of solvent is determined by the number of vaccination doses in the ampoule. Add 0.5 ml to an ampoule with 10 cutaneous doses, and 1 ml of solvent into an ampoule with 20 cutaneous doses. The ampoule is shaken until a homogeneous suspension is formed. The dissolution time of the vaccine should not exceed 5 minutes. The diluted vaccine from an opened ampoule, stored under aseptic conditions, can be used within 4 hours. Vaccination is carried out on outer surface middle third of the shoulder. The vaccination site is treated with ethanol or a mixture of ethanol and ether. The use of other disinfectant solutions is not permitted. After the ethanol and ether have evaporated, use a sterile tuberculin syringe with a thin and short needle (No. 0415), without touching the skin, apply one drop (0.025 ml) of the diluted vaccine to 2 places of future incisions at a distance of 3-4 cm. The skin is slightly stretched and sterile Using a smallpox vaccination pen, 2 parallel cuts 10 mm long are made through each drop of vaccine so that they do not bleed (blood should appear only in the form of small “dewdrops”). Using the flat side of a smallpox vaccination feather, rub the vaccine into the incisions for 30 seconds and allow to dry for 5-10 minutes. A separate disposable feather is used for each person being vaccinated. It is prohibited to use needles, scalpels, etc. instead of pens.
S.C.: Immediately before use, the vaccine is resuspended in 1 ml of a sterile 0.9% NaCl solution. The ampoule is shaken until a uniform suspension is formed. The contents of the ampoule are transferred with a sterile syringe into a sterile vial with 0.9% NaCl solution. In the case of using an ampoule containing 200 subcutaneous vaccination doses, the suspension is transferred to a bottle with 99 ml, and one containing 100 subcutaneous vaccination doses - into a bottle with 49 ml of solvent.
With the syringe method, the vaccine is injected into the area of ​​the lower corner of the scapula in a dose of 0.5 ml. The skin at the injection site is treated with ethanol or a mixture of ethanol and ether. A disposable syringe and needle are used for each person vaccinated. Before each vaccine collection, the vial is shaken. The injection site is lubricated with 5% tincture of iodine.
When using the vaccine subcutaneously using the needle-free method, the vaccine is administered in a volume of 0.5 ml into the area of ​​the outer surface of the upper third of the arm using a needle-free injector with a protector, strictly following the instructions for their use.
The vaccine injection site is treated before and after injection, as with the syringe method.

In history, this disease is known as “sacred fire”, “Persian fire” and other similar associations. It received its modern name because of its distribution area. Today, cases of human infection are casuistic. The disease occurs only in The causative agent was described at the end of the nineteenth century

Pathogen

The bacterium Bacillus anthracis is the cause of anthrax. This is a large stick, Gram-stained purple. It contains a somatic antigen and also secretes a toxin that provokes swelling, interacts with the membranes of body cells and causes death. In addition, the capsule exhibits antiphagocytic properties.

Outside the host body, bacteria form spores that are resistant to exposure high temperature, drying and disinfection. In this form, anthrax can be stored for years and can also be used as a bacterial weapon. Vegetative forms of microorganisms die from exposure hot water after forty minutes, in a dry-heat oven - after 2-3 hours.

Epidemiology

The carriers of the infection are herbivores. As a rule, these are cows, horses, camels or pigs. Infectiousness for humans remains throughout the duration of the disease in livestock, since it secretes the pathogen in environment. And animal carcasses remain infectious for a week. In addition, transmission of the pathogen is realized through blood-sucking insects. Products obtained from sick animals (skin, wool) are especially dangerous. They can remain infective for many years, even after chemical and heat treatment.

Geographically, anthrax is found in countries with a hot, humid climate, in areas where large animals are raised cattle. The disease is usually registered in the summer-autumn period. main reason infection of people - ignoring sanitary and epidemiological standards, as well as insufficient processing of materials before processing.

Pathogenesis and symptoms

Bacteria enter the human or animal body through the skin, mucous membranes or gastric tract. At the “gate of infection”, serous-hemorrhagic inflammation develops, which is manifested by swelling, bleeding and dry necrosis. This place has the appearance of a smoldering coal - a black area bordered by an inflammatory shaft, in the center of which is located open wound. Macrophages carry the pathogen throughout lymphatic system, including to regional lymph nodes. After bacteria enter the blood, secondary sepsis develops with generalization of infection throughout the body: in the lymph nodes, gastrointestinal tract, lungs. Multiple hemorrhages and bleeding appear in target organs.

The incubation period of infection can last from several hours to two weeks, depending on the form. The most common skin manifestations of the disease can be found. At the site of pathogen penetration, red papules and a feeling of itching and burning appear. A day later, the papule evolves into a vesicle filled with serous fluid. After a short time, the contents acquire a crimson or dark purple hue. Because of severe itching a person damages the dome of the vesicle and an ulcer appears with a dark bottom and a bright outline. Bacterial screenings (“necklace symptom”) are formed along its edges, which go through the same stages.

After a couple of weeks, the bottom of the ulcer is covered with a black scab, and a local anesthetic reaction appears for the entire period of the disease. This is one of diagnostic signs anthrax. From common symptoms emit a prolonged fever, accompanied by headache, adynamia and muscle pain. After two to three weeks, local manifestations disappear, and with them the general intoxication symptom.

Diagnostics

The most common cutaneous form of the disease is differentiated from other bacterial boils and carbuncles. Main hallmark is the appearance of a “necklace”, which is characteristic only of anthrax. The generalized form is characterized by a rapid onset, severe intoxication, pulmonary edema, oligo- and anuria. Sometimes, if there is a source of the disease, the doctor needs to distinguish anthrax from tularemia and plague.

From laboratory research To diagnose the disease, you can use bacterioscopy of blood or urine, as well as sowing discharge from a wound onto nutrient media. In addition, laboratory animals can be used to produce more accurate diagnosis. will help determine the phase of the disease and readiness immune system. Another method is to conduct allergy tests with anthraxin.

Anthrax vaccine

To prevent anthrax, a live dry vaccine has been developed that is suitable for subcutaneous or cutaneous use. It is produced in ampoules of 1 ml (calculated for 200 doses subcutaneous administration) complete with 1.5 ml of solvent (glycerin solution).

The anthrax vaccine for humans is a mixture of live spores of the pathogen and purified antigens attached to aluminum hydroxide. The doctor adds an isotonic saline solution to the ampoule, turning the powder into a homogeneous mixture. The shelf life of the dry vaccine is 3 years, and the liquid one - 2.

For adults and children over 1 year of age, any anthrax vaccine can be used. The instructions accompanying it claim that a person develops intense immunity.

Contraindications to vaccination

There are general rules for the administration of all vaccines:

• the patient must be healthy;
• body temperature is in the normal range;
• More than a month has passed since the last ARVI;
• no allergic reactions to previous vaccine administration were observed.

The instructions for using the anthrax vaccine highlight several more contraindications. Thus, a person should not have a history of systemic diseases connective tissue, recurrent dermatological pathologies, as well as problems with the endocrine system. In addition, you need to adhere to time intervals. There should be a gap of at least thirty days between the last vaccination and the anthrax immunization.

Reactions to vaccine administration

The use of anthrax vaccine causes local and general reactions. When administered cutaneously, within 24 hours an area of ​​hyperemia and slight tissue swelling appears on the skin at the injection site. Later, a crust forms there, which disappears over time. Subcutaneous administration of the vaccine is accompanied by the appearance of an infiltrate up to 0.5 cm in size.

General malaise develops extremely rarely and manifests itself in headaches, increased body temperature to subfebrile levels, and enlarged lymph nodes. Sometimes nausea and vomiting are possible. In people with a heightened immune response, the anthrax vaccine may cause allergic reaction up to anaphylactic shock.

Directions for use and dosage

For the first time, vaccination is carried out with dry and liquid vaccine, and subsequently only the dry version is used. As planned, in the first quarter of the year, the susceptible contingent receives a dose of the drug in a volume of up to 0.5 ml subcutaneously.

There are two ways to administer dry anthrax vaccine to humans. The instructions state that children over 14 years of age need to undergo primary immunization subcutaneously in two doses with a break of a month. For adults, apply 2 drops of diluted vaccine to the outer middle third of the shoulder, then scratch the skin and rub in the liquid for half a minute.

In case of contact of an unvaccinated person with a sick animal, he is given emergency prophylaxis in the form of a course of antibiotics:

• for adults it is Ciprofloxacin or Doxycycline;
• for children - Amoxicillin.

Anthrax vaccines for animals

The anthrax vaccine causes the animal to produce antibodies to the pathogen within 10 days from the moment of administration and provides lasting immunity for up to one year. It is intended for routine compulsory immunization of all farm livestock.

For the first time, vaccinations are given in one month old, and then again after six months. Adults should be vaccinated every year. If an animal is found in the livestock population that has signs of anthrax, then the entire herd is vaccinated again.

Contraindications to animal vaccination:

• elevated temperature;
• pregnancy or recent castration;
• surgical operations;
• foals up to 9 months.

The anthrax vaccine is not administered during hot or cold seasons, or in combination with other medications. Treatment with antibiotics, anthelmintic and insectoacaricidal drugs must wait at least 10 days from the date of vaccination. Milk from vaccinated cows can be drunk the very next day after vaccination, but the slaughter of cattle for meat will have to be postponed for two weeks.

Anthrax refers to infectious disease accompanied by a severe course. It develops mainly as a cutaneous form. To prevent its spread, it is necessary to treat anthrax to a certain group of people.

Indications for anthrax vaccination in humans

This vaccination is administered in two cases: planned and epidemic indications.

Scheduled administration of the vaccine is carried out:

• persons involved in the slaughter of livestock, as well as transportation, collection, storage and sale of animal meat;
• people working in a laboratory with live cultures of anthrax bacilli, including those engaged in research on infected animals and materials;
• veterinarians;
• persons work activity which is associated with the processing of leather and wool.

Vaccination usually takes place in the first quarter of each year.

Composition and principle of action of live dry anthrax vaccine STI

The vaccine contains:

• lyophilized suspension of live spores of the bacillus anthracis strain STI-1;
• purified anthrax antigen;
• aluminum hydroxide gel;
• stabilizer, represented by an aqueous solution of sucrose 10%.

The ampoules contain a porous gray-white mass with a tint Brown. Anthrax vaccine is presented in the form of a vacuum-dried suspension of spores of the STI-1 strain (STI – Sanitary-Technical Institute, where the vaccine was developed).

For its production, a persistent type of anthrax bacilli is used, which cannot cause disease in humans. The vaccination is done twice with an interval of 20 to 30 days, thereby forming stable specific immunity, which is formed on the seventh day after vaccination and is valid for one year.

Instructions for use of anthrax vaccine for humans

Before use, each ampoule must be checked for damage.

The vaccine is administered in two ways: cutaneous and subcutaneous:

Contraindications to the introduction of preventive vaccination

There is a range of contraindications that limit the use of the vaccine:

• acute form of infectious and non-infectious diseases. In this case, the vaccine is allowed to be administered only one month after complete recovery;
• recurrent skin diseases;
• pathology endocrine system;
• a history of both primary and secondary immunodeficiencies;
• pregnancy and breastfeeding.

Before vaccination, it is necessary to visit a doctor who will examine the patient to exclude contraindications, as well as thermometry.

Side effects and complications

In the first days after the vaccine is administered, lethargy and headaches may appear, including an increase in body temperature to 38.5°. Slightly swollen lymph nodes may also be present.

In rare cases, it may cause local manifestations, which depend on individual characteristics body:

• after 1-2 days, redness or infiltration may appear. Yellow crusts also appear in the area of ​​the notches;
• Within the same period, pain may occur.

These reactions are short-term and go away on their own without additional treatment.

If your body reacts to the vaccine, you should consult your doctor to clarify the condition.

Price and where to do it

Routine vaccine administration should be free of charge. Vaccination is administered only in medical institutions.

Anthrax vaccine live dry

If vaccination concerns animals, then the vaccination can be done as in veterinary clinic, and called a veterinarian to the house. Especially when it comes to cattle. The price of the drug depends on the dosage. On average in Russia, the cost of the drug for 100 doses starts from 1000 rubles and above.

Vaccine to prevent anthrax

Live anthrax vaccine: Pharmacological action

After two-time use with an interval of 20-30 days, it causes the formation of intense immunity lasting up to 1 year.

Live anthrax vaccine: Indications

Specific prevention of anthrax: persons working with live cultures of the anthrax pathogen, with infected laboratory animals or conducting research on materials contaminated with the anthrax pathogen; persons slaughtering livestock, engaged in the procurement, collection, storage, transportation, processing and sale of raw materials of animal origin; persons performing work in areas enzootic for anthrax (maintenance of public livestock, agricultural, agro- and land reclamation, construction and other work related to the excavation and movement of soil, procurement, fishing, geological, survey, expeditionary work).

Live anthrax vaccine: Contraindications

Acute (infectious and non-infectious) diseases (vaccination is carried out no earlier than 1 month after recovery or remission), immunodeficiency (primary and secondary), simultaneous use of corticosteroids, antimetabolites, radiation therapy(vaccination is carried out no earlier than 6 months after the end of therapy), malignant neoplasms and malignant blood diseases, systemic diseases connective tissue, common recurrent skin diseases, diseases of the endocrine system, pregnancy, lactation, childhood(up to 14 years old).

Live anthrax vaccine: Side effects

When applied cutaneously, a local reaction appears after 24-48 hours: hyperemia, a slight infiltrate, followed by the formation of a yellowish crust along the incisions. With syringe and needleless methods of administration, after 24-48 hours at the injection site there is pain, hyperemia, and less often - an infiltrate with a diameter of up to 50 mm. A general reaction during cutaneous and subcutaneous administration of the vaccine rarely occurs in the first day after administration: malaise, headache, slight increase in temperature. Sometimes there may be an increase in body temperature up to 38.5 degrees C and a slight increase in regional lymph nodes.

Live anthrax vaccine: Method of administration and dose

Cutaneously (scarification) and subcutaneously. It is advisable to carry out unscheduled vaccination subcutaneously. Primary immunization is carried out twice with an interval of 20-30 days, revaccination is carried out once annually. A cutaneous dose of the vaccine is 0.05 ml (contains 500 million spores), one subcutaneous dose is 0.5 ml (50 million spores). Cutaneously (scarification): immediately before use, the contents of the ampoule are resuspended in a sterile 30% aqueous solution of glycerol, which is added to the ampoule using a syringe with a needle for intramuscular administration. The volume of solvent is determined by the number of vaccination doses in the ampoule. Add 0.5 ml to an ampoule with 10 cutaneous doses, and 1 ml of solvent into an ampoule with 20 cutaneous doses. The ampoule is shaken until a homogeneous suspension is formed. The dissolution time of the vaccine should not exceed 5 minutes. The diluted vaccine from an opened ampoule, stored under aseptic conditions, can be used within 4 hours. Vaccination is carried out on the outer surface of the middle third of the shoulder. The vaccination site is treated with ethanol or a mixture of ethanol and ether. The use of other disinfectant solutions is not permitted. After the ethanol and ether have evaporated, use a sterile tuberculin syringe with a thin and short needle (N 0415), without touching the skin, apply one drop (0.025 ml) of the diluted vaccine to 2 places of future incisions at a distance of 3-4 cm. The skin is slightly stretched and sterile Using a smallpox vaccination pen, make 2 parallel cuts 10 mm long through each drop of vaccine so that they do not bleed (blood should appear only in the form of small ones). Using the flat side of a smallpox vaccination feather, rub the vaccine into the incisions for 30 seconds and allow to dry for 5-10 minutes. A separate disposable feather is used for each person being vaccinated. It is prohibited to use needles, scalpels, etc. instead of pens. S.C.: Immediately before use, the vaccine is resuspended in 1 ml of a sterile 0.9% NaCl solution. The ampoule is shaken until a uniform suspension is formed. The contents of the ampoule are transferred with a sterile syringe into a sterile vial with 0.9% NaCl solution. In the case of using an ampoule containing 200 subcutaneous vaccination doses, the suspension is transferred to a bottle with 99 ml, and one containing 100 subcutaneous vaccination doses - into a bottle with 49 ml of solvent. With the syringe method, the vaccine is injected into the area of ​​the lower corner of the scapula in a dose of 0.5 ml. The skin at the injection site is treated with ethanol or a mixture of ethanol and ether. A disposable syringe and needle are used for each person vaccinated. Before each vaccine collection, the vial is shaken. The injection site is lubricated with 5% tincture of iodine. When using the vaccine subcutaneously using the needle-free method, the vaccine is administered in a volume of 0.5 ml into the area of ​​the outer surface of the upper third of the arm using a needle-free injector with a protector, strictly following the instructions for their use. The vaccine injection site is treated before and after injection, as with the syringe method.

Live anthrax vaccine:: Special instructions

It is strictly forbidden to administer subcutaneously a vaccine diluted for cutaneous use! In order to identify contraindications, the doctor (paramedic) on the day of vaccination conducts a survey and examination of the vaccinated with mandatory thermometry. In each individual case, for diseases not included in this list of contraindications, vaccination is carried out only with the permission of the relevant medical specialist. Vaccination is routinely carried out by the cutaneous method in the first quarter of the year, because The most dangerous period in terms of anthrax infection in disadvantaged areas is the spring-summer season. Vaccination is carried out by the average medical staff under the guidance of a doctor. Before use, each ampoule of vaccine is carefully inspected. The vaccine cannot be used if the integrity of the ampoule is damaged or if the appearance dry and dissolved drug (foreign particles, unbroken lumps and flakes), lack of label, expired, in violation of storage conditions. Unused vaccine, used vaccination disposable syringes and feathers are subject to mandatory inactivation by autoclaving at a temperature of (132+2) degrees C and a pressure of 2 kG/sq.m for 90 minutes. Parts of the needleless injector that came into contact with the vaccine, after pretreatment, are immersed in a 6% solution of hydrogen peroxide with 0.5% detergent type or for 1 hour at a temperature not lower than 50 degrees C. The solution is used once. Parts of the injector are sterilized by autoclaving at a temperature of (132+2) degrees C and a pressure of 2 kgf/sq.m for 90 minutes.

Live anthrax vaccine:: Interaction

The interval between vaccination against anthrax and the administration of other vaccines should be at least 1 month.

Dosage form:  lyophilisate for preparing a suspension for subcutaneous administration and cutaneous scarification Compound:

Live anthrax vaccine, lyophilisate for preparing a suspension for subcutaneous administration and cutaneous scarification, is a lyophilized suspension of live spores of the vaccine strain Bacillus anthracis STI-1.

Stabilizer - 10% sucrose solution.

Description: A porous mass of grayish-white or yellowish-white color with a brownish tint. Pharmacotherapeutic group: MIBP - ATX vaccine:  

J.07.A.C Vaccine to prevent anthrax

J.07.A.C.01 Anthrax antigen

Pharmacodynamics:

Live anthrax vaccine after two-time use with an interval of 20-30 days causes the formation specific immunity lasting up to 1 year.

Indications:

Specific prevention of anthrax in people over 14 years of age. Vaccination is carried out as planned and according to epidemic indications.

The following are subject to routine vaccinations:

Persons working with live cultures of the causative agent of anthrax, with infected laboratory animals, or conducting research on materials contaminated with the causative agent of anthrax;

Persons who slaughter livestock, engaged in the procurement, collection, storage, transportation, processing and sale of raw materials of animal origin;

Persons performing the following work in anthrax-enzootic areas:

Public livestock maintenance;

Agricultural, agro-and drainage, construction and other work related to the excavation and movement of soil;

Procurement, fishing, geological, survey, expedition.

Vaccination is routinely carried out in the first quarter of the year, because The most dangerous period in terms of anthrax infection in disadvantaged areas is the spring-summer season.

Contraindications:

1. Acute infectious and non-communicable diseases- vaccinations are carried out no earlier than 1 month after recovery (remission).

2. Primary and secondary immunodeficiencies. When treating with steroids, antimetabolites, or radiotherapy, vaccinations are carried out no earlier than 6 months after the end of therapy.

3. Malignant neoplasms and malignant blood diseases.

4. Systemic connective tissue diseases.

5. Common recurrent skin diseases.

6. Diseases of the endocrine system.

7. Pregnancy and lactation.

In each individual case, for diseases not included in this list, vaccination is carried out only by permission. the relevant medical specialist.

In order to identify contraindications, the doctor (paramedic) on the day of vaccination conducts a survey and examination of the vaccinated with mandatory thermometry.

Directions for use and dosage:

Vaccination is carried out by nursing staff under the guidance of a doctor.

Routine vaccination. Primary immunization is carried out by scarification twice with an interval of 20-30 days, revaccination is carried out once annually by subcutaneous method.

Vaccination according to epidemic indications carried out subcutaneously. If necessary, revaccination is carried out once annually subcutaneously.

Before use, each ampoule of vaccine is carefully inspected. The vaccine cannot be used if the integrity of the ampoule is damaged, the appearance of the dry and dissolved drug changes (the presence of foreign inclusions, unbreakable lumps and flakes), the label is missing, the expiration date has expired, or storage conditions are violated.

1. Vaccination by cutaneous (scarification) method. Based on the number of vaccination doses, the contents of the ampoule (vial) immediately before use are resuspended in a solvent - a sterile 30% aqueous solution of glycerol using a syringe with a needle for intramuscular injection(No. 0840). Add 0.5 ml to an ampoule (bottle) with 10 cutaneous doses, and 1.0 ml of solvent with 20 cutaneous doses and shake until a homogeneous suspension of grayish-white or yellowish-white color with a brownish tint is formed. The dissolution time of the vaccine should not exceed 5 minutes. The diluted vaccine, stored under aseptic conditions, can be used within 4 hours.

The grafting is carried out on the outer surface of the middle third of the shoulder. The grafting site is treated with 70% alcohol. The use of other disinfectant solutions is not permitted. After the alcohol has evaporated, use a sterile tuberculin syringe with a thin and short needle (No. 0415), without touching the skin, apply one drop (0.025 ml) of the diluted vaccine to 2 places of future incisions at a distance of 3-4 cm on the horizontal surface of the shoulder. The skin is slightly stretched and with a sterile smallpox vaccination pen, 2 parallel cuts are made through each drop of vaccine (at a distance of 3-5 mm) length 10 mm in such a way that they do not bleed (blood can only appear in the form of small dewdrops). Using the flat side of a smallpox vaccination feather, rub the vaccine into the incisions for 30 seconds and allow to dry for 5-10 minutes. A separate disposable feather is used for each person being vaccinated.

It is prohibited to use needles, scalpels, etc. instead of feathers.

2. Subcutaneous vaccination.

Immediately before use, the drug is resuspended in 1 ml of sterile sodium chloride solution of 0.9%. The ampoule (bottle) is shaken until a uniform suspension of grayish-white or yellowish-white color with a brownish tint is formed. The contents of the ampoule (vial) are transferred with a sterile syringe into a sterile vial with sodium chloride solution 0.9% for injection. In the case of using an ampoule (bottle) containing 200 subcutaneous vaccination doses, the suspension is transferred to a bottle with 99 ml, and containing 100 subcutaneous vaccination doses - into a bottle with 49 ml of solvent.

With the syringe method the vaccine in a volume of 0.5 ml is injected subcutaneously into the area of ​​the lower angle of the scapula. The skin at the injection site is treated 70% alcohol. Before each vaccine collection, the vial is shaken. The injection site is lubricated with 5% tincture of iodine.

With the needleless method the vaccine in a volume of 0.5 ml is administered into the area of ​​the outer surface of the upper third of the shoulder using a needle-free injector with a protector, strictly following the instructions for its use. The vaccine injection site is treated before and after injection, as with the subcutaneous method.

Unused vaccine, used vaccination disposable syringes and feathers are subject to mandatory inactivation by autoclaving at a temperature of (132±2) °C and a pressure of 2.0 kgf/m2 for 90 minutes.

Parts of the needleless injector that come into contact with the vaccine are immersed in 6 % hydrogen peroxide solution with 0.5% detergent type "Progress" or "Astra" for 1 hour at a temperature not lower than 50 ° C. The solution is used once. Then the injector is pre-sterilized:

a) rinsing under running water for 0.5 minutes;

b) soaking at full immersion into the washing solution at a temperature of 50 °C for 15 minutes. Recipe for 1 liter of washing solution: 17 g perhydrol (27.5 g 33% hydrogen peroxide), 5 g of detergent and 978 ml of water;

c) washing each item in a washing solution using a brush or a cotton-gauze swab for 0.5 minutes;

d) rinsing under running water for 10 minutes;

e) rinsing each item with distilled water for 0.5 minutes;

f) drying until moisture completely disappears.

Sterilization of parts of a needleless injector is carried out by autoclaving at temperature (132±2) °C and pressure2.0 kgf/m2 for 90 minutes.

Reaction to introduction

Vaccine vaccinations may be accompanied by local reactions, the intensity of which depends on the individual characteristics of those vaccinated. 24-48 hours after cutaneous vaccination, hyperemia and infiltration may occur at the site of vaccine administration, followed by the formation of crusts along the incisions yellowish color. 24-48 hours after subcutaneous vaccination, pain, hyperemia, and, less commonly, an infiltrate with a diameter of up to 50 mm may occur at the injection site.

Side effects:

It may manifest itself in the first day after vaccination with malaise, headache and an increase in body temperature up to 38.5 ° C.

Interaction: Interval between vaccinesinaziagainst anthrax and other vaccines must be at least one month old, and for children - at least two months.

The vaccine is sensitiveToantibiotics, and therefore immunization while using antibiotics is not allowed.

Release form/dosage:Lyophilisate for preparing a suspension for subcutaneous administration and cutaneous scarification. Package:

200 subcutaneous or 20 cutaneous vaccination doses in an ampoule (vial) (solvent - 30% glycerol solution, 1.5 ml in an ampoule) or 100 subcutaneous or 10 cutaneous vaccination doses in an ampoule (vial) (solvent - 30% glycerol solution, 1.5 ml each) 0 ml per ampoule).